characterization-immune-cells-human-adipose-tissue-using-flow

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Infiltration of immune cells in the subcutaneous and visceral adipose tissue (AT) deposits leads to a low-grade inflammation contributing to the ...

Immune cell accumulation in adipose tissue (AT) is associated with the development of AT inflammation, resulting in metabolic dysfunction. Circulating immune cell patterns may reflect immune cell accumulation in expanding AT. However, data linking human leukocytes in blood and AT is lacking. We investigated whether blood immune cell populations are associated with their counterparts in subcutaneous (scAT) or visceral AT (vAT). Flow cytometry was performed on blood, scAT and vAT from 16 lean and 29 obese men. Circulating natural killer (NK)-cells, classical monocytes and nonclassical monocytes were higher in obese individuals. vAT, but not scAT, of obese individuals contained more inflammatory CD11c"M1" macrophages and NK cells compared to lean individuals. Blood classical monocytes were associated with CD11cmacrophages in vAT but not scAT. This association was unrelated to expression of the adhesion molecules CD11b and CD11c or of the chemokine receptor CX3CR1 on these monocytes. Other AT immune cells were not associated with their respective counterparts in blood. Finally, CD11cmacrophages and CD4T-cells in vAT were associated with their counterparts in scAT. In conclusion, blood classical monocytes reflect CD11cmacrophages in vAT.

Circulating classical monocytes are associated with CD11cmacrophages in human visceral adipose tissue.

Obesity is a risk factor for non-alcoholic steatohepatitis (NASH). This risk has been attributed to visceral adipose tissue (vAT) expansion associated with increased proinflammatory mediators. Accumulation of CD11cproinflammatory adipose tissue macrophages (ATM) is an important driver of vAT inflammation. We investigated the role of ATMs in hepatic inflammation during NASH development.

Article	Year
Circulating classical monocytes are associated with CD11cmacrophages in human visceral adipose tissue. Scientific reports\| PubMed ID: 28198418	2017
Adipose tissue macrophages induce hepatic neutrophil recruitment and macrophage accumulation in mice. Gut\| PubMed ID: 29074725	2017

Mitchell Bijnen

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