study-on-metabolism-six-systemic-insecticides-newly-established-cell

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (<em>Camellia Sinensis </em>L.) Leaves

A platform for studying insecticide metabolism using in vitro tissues of tea plant was developed. Leaves from sterile tea plantlets were induced to form ...

This review summarizes the isolation, chemical identification, and biochemical activities of Camellia triterpenoid saponins, updating a previous review and encompassing all new studies through September 2017. Further, molecular simulations of the interaction between several known cytotoxic oleiferasaponin monomers and Interleukin-6 are discussed, demonstrating that molecular modeling is a convenient method to obtain structure-activity information.

Triterpenoid saponins from the genus Camellia: structures, biological activities, and molecular simulation for structure-activity relationship.

An efficient method for the rapid extraction and clean-up of 20 pesticide residues from polyphenol-rich agricultural samples (tea, apple, broccoli, and shallot) for analysis by UPLC-MS/MS was developed. Following extraction, PVPP was used to precipitate polyphenols, supernatant was diluted to minimize matrix effects. Homogenized samples were vortexed in acetonitrile prior to cleanup with a combination of PVPP (150 mg), PSA (50 mg) and GCB (10 mg). Supernatant (1 mL) was filtered and diluted 10-fold before analysis. In 4 agricultural products that usually produce high interference, the matrix effects were overcome for all pesticides expect in green and oolong tea for acephate, omethoate, dinotefuran and nitenpyram. Mean recoveries ranged from 73% to 106%, and RSD ≦ 13%. Limits of quantification ranged from 0.01 to 0.02 mg kg. PVPP as one of excellent QuEChERS material combined with dilution was verified as a promising method for multiple pesticide residues analysis in complex matrices.

Analysis of multiple pesticide residues in polyphenol-rich agricultural products by UPLC-MS/MS using a modified QuEChERS extraction and dilution method.

We have previously demonstrated that several new saponins from the seed cake of Camellia oleifera Abel. exhibited antiproliferative activity against human tumor cells in vitro. The current study investigated the effect of total saponins from the residual seed cake of Camellia oleifera Abel. (TSSC) on anticancer activity in hepatoma-22 tumor-bearing mice and discovered that TSSC induced apoptosis of cancer cells in mice with hepatoma-22 solid tumors. In mice with hepatoma-22 solid tumors, daily intratumoral injections with TSSC at the doses of 20 μg kg-1, 100 μg kg-1, or 2000 μg kg-1 were administered for 10 consecutive days, a regimen which was well tolerated by the mice and significantly inhibited tumor growth. Moreover, TSSC promoted solid tumor cell apoptosis, upregulated the protein expression of Bax, and downregulated the protein expression of Bcl-2 in response to regulate apoptosis of cancer cells in mice bearing hepatoma 22 solid tumors. At the same time, the direct structure-activity relationship between camelliasaponins B1, Bcl-2 and MDM2 in TSSC was investigated by molecular docking. It was verified that the glycosidic ligand on C3 is the main source of anticancer activity. Taken together, these results indicated that TSSC could exhibit anticancer activity and increase apoptosis of cancer cells in hepatoma-22 tumor-bearing mice, making it a potential adjuvant drug after further investigation in the future.

Article	Year
Triterpenoid saponins from the genus Camellia: structures, biological activities, and molecular simulation for structure-activity relationship. Food & function\| PubMed ID: 29888775	2018
Analysis of multiple pesticide residues in polyphenol-rich agricultural products by UPLC-MS/MS using a modified QuEChERS extraction and dilution method. Food chemistry\| PubMed ID: 30372964	2019
Anticancer activity and mechanism of total saponins from the residual seed cake of Camellia oleifera Abel. in hepatoma-22 tumor-bearing mice. Food & function\| PubMed ID: 30977498	2019

Ruyan Hou

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