analysis-iophenoxic-acid-analogues-small-indian-mongoose-herpestes

Analysis of Iophenoxic Acid Analogues in Small Indian Mongoose (<em>Herpestes Auropunctatus</em>) Sera for Use as an Oral Rabies Vaccination Biological Marker

The small Indian mongoose (Herpestes auropunctatus) is a reservoir of rabies virus (RABV) in Puerto Rico and comprises over 70% of animal rabies cases ...

A safety requirement for live vaccines is investigating possible shedding in recipients since the presence of replication competent vaccine in secretions could result in direct and indirect horizontal transmission. This is especially relevant for oral rabies vaccine baits that are deliberately distributed into the environment. In the current study, survival of an oral rabies virus vaccine, SPBN GASGAS, was examined in excretions from different target and non-target species; red fox, raccoon dog, small Indian mongoose, raccoon, striped skunk, domestic dog, domestic cat and domestic pig. Saliva - and (pooled) fecal samples collected at different time points after oral administration of the vaccine strain were examined for the presence of viral RNA (rt-PCR). All PCR-positive and a subset of PCR-negative samples were subsequently investigated for the presence of infectious virus by isolation in cell culture (RTCIT). Up to 7 days post vaccine administration viral RNA could be detected in 50 of 758 fecal samples but no infectious virus was detected in any of the examined PCR-positive fecal samples. In contrast, RNA-fragments were detected in 248 of 1053 saliva swabs for an extended period (up to 10 days) after vaccine administration, but viable virus was only present during the first hours post vaccine administration in 38 samples. No infectious vaccine virus was isolated in saliva swabs taken 24 h or more after vaccine administration. Hence, no active shedding of the vaccine virus SPBN GASGAS after oral administration occurred and the virus isolated during the initial hours was material originally administered and not a result of virus replication within the host. Thus, potential horizontal transmission of this vaccine virus is limited to a short period directly after vaccine bait uptake. It can be concluded that the environmental risks associated with shedding after distributing vaccine baits containing SPBN GASGAS are negligible.

An assessment of shedding with the oral rabies virus vaccine strain SPBN GASGAS in target and non-target species.

Oral vaccination of the small Indian mongoose against rabies has been suggested as a potential tool to eliminate mongoose-mediated rabies on several Caribbean islands. A recently developed oral rabies virus vaccine strain, SPBN GASGAS, has already been shown to be efficacious in this reservoir species. Since, all available oral rabies vaccines are based on replication-competent viruses and vaccine baits are distributed unsupervised in the environment, enhanced safety standards for such vaccine types are required.

Safety studies with the oral rabies virus vaccine strain SPBN GASGAS in the small Indian mongoose (Herpestes auropunctatus).

(1) Background: As part of the ongoing endeavor to eliminate dog-mediated human rabies in Thailand, renewed interest has been shown in oral vaccination of dogs as a supplementary tool to increase vaccination coverage of the dog population. (2) Methods: Three different bait types were tested using a hand-out model on the campus of the Kasetsart University and the surrounding temples in Thailand during September 2017, consisting of two industrial manufactured baits (fish meal and egg-flavored) and one bait made from local material (boiled pig intestine placed in collagen casing). A PVC-capsule containing dyed water was inserted in the bait. (3) Results: The fishmeal bait was significantly less often accepted and consumed (50.29%) than the other two baits (intestine bait&mdash;79.19%; egg bait&mdash;78.77%). Delivery and release of the dyed water in the oral cavity was highest in the egg-flavored bait (84.50%), followed by the intestine bait (76.61%) and fishmeal (54.85%) baits. Bait acceptance was influenced by sex, age, and body size of the dog. Also, the origin of the dogs had a significant effect: temple dogs accepted the baits more often than street dogs. (4) Conclusion: A significant portion of the free-roaming dog population in this study can be vaccinated by offering vaccine baits.

Article	Year
An assessment of shedding with the oral rabies virus vaccine strain SPBN GASGAS in target and non-target species. Vaccine\| PubMed ID: 29325820	2018
Safety studies with the oral rabies virus vaccine strain SPBN GASGAS in the small Indian mongoose (Herpestes auropunctatus). BMC veterinary research\| PubMed ID: 29534727	2018
Field Studies Evaluating Bait Acceptance and Handling by Free-Roaming Dogs in Thailand. Veterinary sciences\| PubMed ID: 29734697	2018

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