planarian-ovary-dissection-for-ultrastructural-analysis-antibody

Planarian Ovary Dissection for Ultrastructural Analysis and Antibody Staining

Accessibility to germ cells allows the study of germ cell development, meiosis, and recombination. The sexual biotype of the freshwater planarian, ...

modified-pasm-masson-staining-in-a-renal-biopsy

Modified PASM-Masson Staining in a Renal Biopsy

Special histochemical staining is an important method for a renal biopsy, which includes PAS staining, periodic acid silver methenamine (PASM) staining, ...

the-associations-between-multi-theory-model-mtm-constructs-health

The Associations between Multi-Theory Model (MTM) Constructs and Health Behavior Change among College Students: A Systematic Review and Meta-Analysis

This study aims to explore the role of the Multi-Theory Model (MTM) in health behavior change among college students, to quantify the strength of the ...

Tumor-initiating stem cells (TSCs) are critical for drug resistance and immune escape. However, the mutual regulations between TSC and tumor microenvironment (TME) remain unclear. Using DNA-label retaining, single-cell RNA sequencing (scRNA-seq), and other approaches, we investigated intestinal adenoma in response to chemoradiotherapy (CRT), thus identifying therapy-resistant TSCs (TrTSCs). We find bidirectional crosstalk between TSCs and TME using CellPhoneDB analysis. An intriguing finding is that TSCs shape TME into a landscape that favors TSCs for immunosuppression and propagation. Using adenoma-organoid co-cultures, niche-cell depletion, and lineaging tracing, we characterize a functional role of cyclooxygenase-2 (Cox-2)-dependent signaling, predominantly occurring between tumor-associated monocytes and macrophages (TAMMs) and TrTSCs. We show that TAMMs promote TrTSC proliferation through prostaglandin E2 (PGE2)-PTGER4(EP4) signaling, which enhances β-catenin activity via AKT phosphorylation. Thus, our study shows that the bidirectional crosstalk between TrTSC and TME results in a pro-tumorigenic and immunosuppressive contexture.

ArticleTotal : 3	Year
Publication title Cited by 1	2021
Publication title
Publication title	2025

Xia Zhao

Biography

JoVE Journal Publications

Other Publications