fluorescence-detection-methods-for-microfluidic-droplet-platforms

Fluorescence detection methods for microfluidic droplet platforms

The development of microfluidic platforms for performing chemistry and biology has in large part been driven by a range of potential benefits that ...

Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.

Recent advances in microfluidic technologies for biochemistry and molecular biologys.

Fortschritte in den Bereichen Proteomics und Genomics erforderten die Entwicklung von High-Throughput-Screening-Methoden (HTS) f&uuml;r die systematische Transformation gro&szlig;er Mengen von biologischen und chemischen Daten in eine organisierte Datenbank des Wissens. Mikrofluidische Systeme sind ideal geeignet f&uuml;r Hochdurchsatz-biochemische Experimente, da sie analytische Hochdurchsatz bieten, minuzi&ouml;se Quantit&auml;ten von teuren biologische Reagenzien zu konsumieren, weisen hervorragende Empfindlichkeit und Funktionalit&auml;t im Vergleich zu traditionellen Mikro-Array Techniken und innerhalb komplexer experimenteller Arbeitsabl&auml;ufe integriert werden k&ouml;nnen. Eine Reihe von grundlegenden biochemischen und molekularen biologischen Vorg&auml;nge im letzten Jahrzehnt, inklusive Gensequenzierung Microfluidic Chip-basierten Formaten &uuml;bertragen wurden, emulsion, PCR, Immunoassays, Elektrophorese, zellbasierte Assays, Ausdruck Klonen und Makromolek&uuml;l beflecken. In diesen Bericht beleuchten wir einige der j&uuml;ngsten Fortschritte bei der Anwendung der Mikrofluidik, Biochemie und Molekularbiologie.

Soongwon Cho

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