mass-production-genetically-modified-aedes-aegypti-for-field-releases

Mass Production of Genetically Modified <em>Aedes aegypti</em> for Field Releases in Brazil

New techniques and methods are being sought to try to win the battle against mosquitoes. Recent advances in molecular techniques have led to the ...

Dengue and dengue hemorrhagic fever are increasing public health problems with an estimated 50-100 million new infections each year. Aedes aegypti is the major vector of dengue viruses in its range and control of this mosquito would reduce significantly human morbidity and mortality. Present mosquito control methods are not sufficiently effective and new approaches are needed urgently. A "sterile-male-release" strategy based on the release of mosquitoes carrying a conditional dominant lethal gene is an attractive new control methodology. Transgenic strains of Aedes aegypti were engineered to have a repressible female-specific flightless phenotype using either two separate transgenes or a single transgene, based on the use of a female-specific indirect flight muscle promoter from the Aedes aegypti Actin-4 gene. These strains eliminate the need for sterilization by irradiation, permit male-only release ("genetic sexing"), and enable the release of eggs instead of adults. Furthermore, these strains are expected to facilitate area-wide control or elimination of dengue if adopted as part of an integrated pest management strategy.

Female-specific flightless phenotype for mosquito control.

Denguefieber und h&auml;morrhagisches Denguefieber vermehrt Probleme im Gesundheitswesen mit einer gesch&auml;tzten 50 Millionen Neuinfektionen pro Jahr. Aedes Aegypti ist, dass die gro&szlig;en Vektor von Dengue-Viren in seinem Bereich und Kontrolle dieser M&uuml;cke deutlich menschlicher Morbidit&auml;t und Mortalit&auml;t reduzieren w&uuml;rde. Vorhanden Moskito-Control-Methoden sind nicht hinreichend wirksam und neue Ans&auml;tze werden dringend ben&ouml;tigt. Eine &quot;sterile-M&auml;nnchen-Release&quot;-Strategie basiert auf der Ver&ouml;ffentlichung von Moskitos tragen ein bedingte dominante Lethal gen ist eine attraktive neue Steuerelement-Methode. Transgene St&auml;mme von Aedes Aegypti wurden so konstruiert, dass einen repressible Weibchen-spezifische flugunf&auml;higen Ph&auml;notyp mit zwei separaten transgene oder eine einzelne Transgene, basierend auf der Verwendung von Frauen-spezifische indirekte Flug Muskel F&ouml;rderer von Aedes Aegypti Actin-4 Gen haben. Diese St&auml;mme &uuml;berfl&uuml;ssig Sterilisation durch Bestrahlung, ausschlie&szlig;lich m&auml;nnlich dominierten eingef&uuml;hrt (&quot;genetische sexing&quot;) und aktivieren die Freigabe der Eier statt Erwachsene. Dar&uuml;ber hinaus sollen diese St&auml;mme fl&auml;chendeckende Kontrolle oder Beseitigung von Dengue-Fieber wird angenommen als Teil einer Strategie des integrierten Pflanzenschutzes-Management zu erleichtern.

Weiblich-spezifische flugunf&auml;hige Ph&auml;notyp f&uuml;r Moskito-Kontrolle.

Dengue is the most important mosquito-borne viral disease. In the absence of specific drugs or vaccines, control focuses on suppressing the principal mosquito vector, Aedes aegypti, yet current methods have not proven adequate to control the disease. New methods are therefore urgently needed, for example genetics-based sterile-male-release methods. However, this requires that lab-reared, modified mosquitoes be able to survive and disperse adequately in the field.

Article	Year
Female-specific flightless phenotype for mosquito control. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 20176967	2010
Open field release of genetically engineered sterile male Aedes aegypti in Malaysia. PloS one\| PubMed ID: 22970102	2012

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