Guest Editor
Collection Overview
Due to their unique mode of action, CRISPR-Cas systems employ multiple sophisticated mechanisms. These include numerous interactions between various types of molecules (e.g., RNA, DNA, and proteins). Precise description of these interactions is crucial both for basic studies as well for designing practical applications for CRISPR-Cas systems of various types. Yet, it is not an easy task to adapt existing methods to achieve reliable results in this uneasy field. The aim of this collection is to gather methods developed and optimized for studying CRISPR-Cas systems at each stage of their activity: spacer acquisition, biogenesis, and interference.
Articles
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
0 Views
•
2025
•
1Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, 2Institut de Biologie Paris-Seine (IBPS), UMR CNRS 8263, INSERM U1345, Development, Adaptation and Ageing, Sorbonne Université, 3College of Marine Life Sciences, Ocean University of China, 4Key Laboratory for Experimental Teratology of the Ministry of Education, Shandong University, 5State Key Laboratory of Microbial Technology, Shandong University, 6Shandong University-Yuanchen Joint Biomedical Technology Laboratory