JoVE | Peer Reviewed Scientific Video Journal

TOPICAL COLLECTIONS

Single-Cell Dynamics and Infection

Guest Editors

Nicolas Personnic

Group Persistence and Single-Cell Dynamics of Respiratory Pathogens (PERSIST), Lyon - France

<p>Nicolas Personnic is the founder and leader of the “PERSIST” team (CIRI, Lyon). He is one of the international experts whose research aims at deciphering the molecular mechanisms driving the cell-to-cell variation within a population of bacterial pathogens during the infection, as well as, to reveal the ensuing functional consequences. His work mostly focuses on the bacterial antibiotic persisters.</p>

Paul Vehroeven

University Hospital of St-Etienne, France

<p>Prof. Paul Vehroeven is MD, PhD at the Faculty of Medicine of St-Etienne and at the University Hospital of St-Etienne in France. He is head of the clinical lab of Bacteriology and Hygiene and Chair of Hospital Infection Control Committee at the University Hospital of St-Etienne. He is in charge of teaching microbiology to the students in second year of medical school. He is Co-PI of the GIMAP team (CIRI) located at the faculty of Medicine of St-Etienne. He currently works on host pathogens interactions with staphylococci. His work covers everything from clinical epidemiological studies to more fundamental research on the host response to S. aureus colonization and infection.</p>

Collection Overview

The field of pathogen single-cell dynamics, phenotypic variation, and infection has gained significant attention in recent years due to its potential to unravel the intricate mechanisms underlying infectious diseases, pathogen eco-pathological life cycles, and evolution. In this JoVE Methods Collection, we bring together a diverse range of methods to delve into the fascinating world of pathogen-host interactions at the single-cell level. By employing advanced techniques such as single-cell sequencing, quantitative imaging, and high-throughput technologies, researchers aim to elucidate the dynamic processes occurring during infection and gain insights into host responses, pathogen behavior, and the overall progression of infectious diseases.

This collection invites methods including, but not limited to, the following topics:

Phenotypic plasticity in pathogens
Host cell heterogeneity
Pathogen invasion strategies and host cell interactions
Single-cell profiling of host immune responses
Dynamics of pathogen populations
Single-cell analysis in clinical applications

By employing cutting-edge single-cell technologies, researchers aim to unravel the hidden complexities of infectious diseases, provide a deeper understanding of host-pathogen interactions, and ultimately contribute to the development of innovative therapeutic strategies. The collection will serve as a valuable resource for scientists, clinicians, and students interested in the emerging field of pathogen single-cell dynamics and infection.